By R. O. Morris, D. A. Regier, E. M. S. MacDonald (auth.), Professor Dr. Jean Guern, Professor Dr. Claude Péaud-Lenoël (eds.)
In a few fresh biology conferences, plant development components were a favourite subject. One may perhaps ask if a brand new colloquium dedicated to the metab olism and the actions of cytokinins, thought of on the molecular point, used to be quite invaluable and should upload whatever very important to the former contributions. in truth, the first function of the organizers, of the French nationwide heart of medical examine, and of the nationwide In stitute of Agronomic examine which subsidized the colloquium used to be much less the version of a complaints ebook than the will to collect a few scientists who had made contributions in the direction of fixing prob· lems in cytokinin examine and thereby to supply a chance for fruitful discussions with no regulations within the timetable. certainly this first goal used to be attained. in addition, vital new fmdings and con clusions have been provided via the members that merited book within the shape ofthis publication. it's past the scope of this foreword to adver tize or perhaps to categorise those new fmdings. we will in simple terms point out some of them. The reader will take pleasure in the contributions of these authors who labored tough to clarify the biosynthetic pathways of common cytokin ins.
Read Online or Download Metabolism and Molecular Activities of Cytokinins: Proceedings of the International Colloquium of the Centre National de la Recherche Scientifique held at Gif-sur-Yvette (France) 2–6 September 1980 PDF
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Additional info for Metabolism and Molecular Activities of Cytokinins: Proceedings of the International Colloquium of the Centre National de la Recherche Scientifique held at Gif-sur-Yvette (France) 2–6 September 1980
In the highly virulent MW2, loss of virulence was associated with loss of the 63-Mdal plasmid, but not of the 120-Mdal plaSmid. From these results, it appears that pathogenicity is associated with the presence of plasmids. In strains so far tested both the high i6 Ade content in the culture medium and the presence of high amounts of hydroxylated isopentenyl derivatives (cis- and transzeatin and their derivatives) in the culture medium and of the corresponding zeatin ribonucleotides in tRNA appear to be associated with the presence of the 63-Mdal plasrnids.
Although there is evidence that cytokinins from xylem exudate may come from the roots (see review by Torrey, 1976), the question of whether the intact root tips and/or other plant parts serve as a primary site of cytokinin biosynthesis was not answered. The following experiments were designed to address this question. Rootless tobacco plants were originated from cytokinin-heterotrophic tobacco tissue (Nicotiana tabaccum Var. Wis. No. 38). LM IAA to induce plantlet formation (Skoog and Miller, 1957).
1978) on Lichrosorb RP8 semi-preparative columns (20 x 10 mm id) equilibrated and eluted with H2 O/MeOH (70:30 for the i0 6 ado and i0 6 ade fractions and 40:60 for the i 6 ado and i 6 ade fractions). The column eluates were monitored at 260 nm. The fractions were collected directly into plastic scintillation vials as indicated in the figures. The vials were placed in an oven overnight at 35° to remove most of the eluant.